LAMBS moved to IIT - We are a RESEARCH group of BIOPHYSICISTS merging different backgrounds, namely: physics, bioengineering, biotechnology,materials science, chemistry, biology, medicine,computer science. We work for conceiving, designing, realizing, utilizing methods and techniques to study biological systems to advance life sciences In 1999, thanks to the strong backgorind in 3D optical microscopy Alberto Diaspro, within the strategic framework of a national project of the National Institute of the Physics of Matter (INFM), Section B,designed and realised the first italian two-photon excitation fluorescence microscope, part of a multipurpose architecture including also lifetime imaging and fluorescence correlation spectroscopy modules. The core of the architecture is a mode-locked Ti:Sapphire infrared pulsed laser (Tsunami 3960, Spectra Physics Inc., CA), pumped by a high-power (5W 532 nm) solid state laser (Millennia V, Spectra Physics Inc., CA). The system is endowed of a Lok-to-clock module (model 3930, Spectra Physics) Inc., CA). The features of infrared pulsed laser are high average power, 80 MHz high repetition rate, and 100 fs short pulse width. Tsunami has a tuning range from 650 nm to 1050 nm and in the actual configuration is optimized in a range from 680 nm to 830 nm delivering an output average power from 200 mW to 900 mW.