Focus On Microscopy 2003 ISS
home Research Partners Staff Contacts Links News
explanation links

Staff

PRINCIPAL INVESTIGATORS: Alberto Diaspro
POST-DOC: Raffaella Magrassi - Silke Krol
PHD STUDENTS: Paolo Bianchini - Valentina Caorsi - Davide Mazza - Giuseppe Vicidomini
THESIS STUDENTS: Federica Morotti - Ilaria Testa - Francesca Cella - Mattia Pesce

Alberto Diaspro

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536426, Facsimile: +39-010314218
diaspro@fisica.unige.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, Bioimaging, Molecular and Cell Biophysics, Nanostructured hybrid materials, Nano-bio-robot.

AD (Genoa, Italy, April 7, 1959) is at the University of Genoa in the Applied Physics area. His research aims at the study of biological molecules signals in cells using 3D, confocal and multiphoton fluorescence microscopy, FRET, FRAP, FLIM, single molecule imaging, scanning probe microscopy, polarized light scattering, nano-bio-robot, bioimaging. He joins IFOM (FIRC Foundation, Milan) MicroScoBio (Genoa). He is in the NANOMED FIRB program (2005-2010). A. Diaspro et al., Quart. Rev.Bioph., in press (2005). A.Diaspro in Chirico et al. ChemPhysChem. 6(2):328 (2005). A.Diaspro in Chirico et al., Phys.Rev E, 70:030901 (2004). A.Diaspro in Fabrizio et al. J.Cell Biol. 166 (7):1055 (2004). A. Diaspro et al., J Phys Chem B 107: 11008 (2003). A.Diaspro et al. Langmuir, 18, 5047(2002).


Raffaella Magrassi

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-010314218
magrassi@fisica.unige.it; http://www.lambs.it

Specialty Keywords:

Raffaella Magrassi was born in Genoa, Italy 12/01/1971. She received the degree in Biology from the University of Genoa, Italy, 1997 and the Ph.D degree from the University of Siena, Italy 2003. Currently she is a Post Doctoral Researcher at The National Institute for the Physics of Matter (INFM) -Dept. of Physics, University of Genoa. The focus of her research activities lies on Smart drug delivery, Biomimetic and Nanostructured systems.
Her most noticeable publications are:

  • Krol S., Nolte M., Diaspro A., Mazza D., Magrassi R. et al.(2005) LANGMUIR, 21(2) : 705-709;
  • Krol S., Diaspro A., Magrassi R.et al. (2004) IEEE Transaction on nanobioscience, 3 : 32-38;
  • Pusch, M., Magrassi, Ret al.. (1998) Biophysical Journal, vol.75: 782-792;
  • Zocchi E., Basile G., Cerrano C., Bavestrello G., Giovine M., Bruzzone S., Guida L., Carpaneto A., Magrassi R., and Usai C. (2003) Journal of Cell Science, 116: 629-636.

Silke Krol

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-010314218
krol@fisica.unige.it; http://www.lambs.it

Specialty Keywords: Nanostructured systems, Polyelectrolyte capsules, Targeted drug delivery, diabetes treatment.

SK (Gronau, Germany; September 17, 1968) is Researcher/contract Prof. at the University of Genoa in the field of nanostructured systems and their applications. Her research activity is focused on the development of tailored nanometer-sized or nano-organized systems, mainly nanocapsules for medical or pharmaceutical purpose. Her task is to construct capsules for protective as well as for targeting aspects. Furthermore she investigates the reaction of living cells to the enveloping polyelectrolyte shell.
Krol S., et al. (2004) IEEE Transactions on nanobioscience, 3, 32-38. Krol S., et al. (2003) J. Microsc. 212, 239-243. Krol S., et al. (2005) Langmuir, 21, 705-709.


Paolo Bianchini

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-010314218
bianchini@ge.infm.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, Bioimaging

PB (Genoa, Italy, March 20, 1978) is PhD student at the University of Genoa in the Applied Physics area. His research activity aims at the study of biological molecules to address cell functioning using conventional, confocal and multiphoton fluorescence microscopy, SHG, FLIM, single molecule imaging, scanning probe microscopy, spectral microscopy, nanostructured model systems, bioimaging. He joins IFOM (Institute for Cancer Research, Milan) and MicroScoBio (Research Center for Correlative Microscopy in Biomedicine and Oncology).
2004 GIC(Citometry Italian Society) Massimo Grattarola Award for the thesis about “Study EGFP expression in encapsulated yeast cells”.


Valentina Caorsi

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-010314218
caorsi@fisica.unige.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, Bioimaging

VC (Genoa, Italy, April 30, 1980) is PhD student at the University of Genoa in the Applied Physics area. Her research activity aims at the study of biological molecules to address cell functioning using conventional, confocal and multiphoton fluorescence microscopy. In particular she is involved in FRET analysis on nanostructured model systems.


Davide Mazza

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-0103992355
mazza@fisica.unige.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, Bioimaging, Nanostructured Systems, Diffusion, FRAP.

Davide Mazza (Genoa, Italy, May 29, 1980) is a PhD student at the University of Genoa in the Applied Physics area. His research activity aims at the study of biological molecules to address cell functioning using conventional, confocal and multiphoton fluorescence microscopy, with special attention to diffusion measurements in biological samples and model systems by means of FRAP (Fluorescence Recovery After Photobleaching) technique.
Krol S., Nolte M., Diaspro A., Mazza D., Magrassi R., Gliozzi A., Fery A. Encapsulated living cells on microstructured surfaces. (2005) Langmuir, 21, 705-709.


Giuseppe Vicidomini

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-010314218
vicidomini@fisica.unige.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, Deconvolution

GV (Savona, Italy, November 29, 1978) is Phd Student at the University of Genoa in Computer Science. His research activity aims at the study of process image formation in conventional, confocal and multi-photon excitation fluorescence microscopy. In particular he is involved in deconvolution and in image process (co-localization, FRET analysis). He joins MicroScoBio (Reserach Center for Correlative Microscopy in Biomedicine and Oncology).


Ilaria Testa

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-018563288
ilaria.testa@email.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, Photoactivation, Bioimaging.

IT (Rapallo (Ge), Italy, December 22, 1981) is Thesis Student at the University of Genoa in the Applied Physics area. Her research activity aims at the study of biological molecules to address cell functioning using conventional, confocal and multiphoton fluorescence microscopy, photoactivation, FRAP, FLIM, single molecule imaging, scanning probe microscopy, nanostructured model systems, bioimaging. She joins IFOM (Institute for Cancer Research, Milan) and MicroScoBio (Reserach Center for Correlative Microscopy in Biomedicine and Oncology).


Francesca Cella

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-0103992355
ilaria.testa@email.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, Photoactivation, Bioimaging.

Francesca Cella (Genoa, Italy, April 5, 1980) is thesis student at the University of Genoa in the Applied Physics area. Her research activity aims at the study of biological molecules to address cell functioning using conventional, confocal and multiphoton fluorescence microscopy, FRAP, nanostructured model systems.


Mattia Pesce

LAMBS-MicroScoBio, Dept. of Physics, University of Genoa,
Via Dodecaneso 33, Genoa, Liguria, 16146, Italy
Tel: +39-0103536309, Facsimile: +39-010314218
gattodub@email.it; http://www.lambs.it

Specialty Keywords: Confocal Microscopy, Multiphoton microscopy, FLIM.

MP(Acqui Terme Italy, November 26, 1980) is Thesis Student University of Genoa in the Applied Physics area. His research activity aims at the study of biological molecules to address cell functioning using conventional, confocal and multiphoton fluorescence microscopy, FRET, FLIM, nanostructured model systems, bioimaging.



Some publications among 80 in peer reviewed journals and 380 including conferences
Marc Schneider, Sara Barozzi, Ilaria Testa, Mario Faretta and Alberto Diaspro,
(2005) "Two-photon activation and excitation properties of PA-GFP in the 720-920 nm region",
Biophys. J., vol.89(2).

Biophysical Society Newsletter p.18, March-April 2005 Issue.
G. Chirico, A. Diaspro, F. Cannone, M. Collini, S. Bologna, V. Pellegrini and F. Beltrame,
"Selective Fluorescence Recovery after Bleaching of Single E2GFP Proteins Induced by Two-Photon Excitation",
ChemPhysChem 2005, 6, 328 – 335.
Luca Raiteri, Sara Stigliani, Laura Patti, Cesare Usai, Giovanna Bucci, Alberto Diaspro, Maurizio Raiteri and Giambattista Bonanno,
"Activation of c-Aminobutyric Acid GAT-1 Transporters on Glutamatergic Terminals of Mouse Spinal Cord Mediates Glutamate Release Through Anion Channels and by Transporter Reversal",
Journal of Neuroscience Research 80:424–433 (2005).
G. Chirico, F. Cannone, A. Diaspro, S. Bologna, V. Pellegrini, R. Nifosì and F. Beltram,
"Multiphoton switching dynamics of single green fluorescent proteins",
PHYSICAL REVIEW E 70, 030901(R) (2004).
Silke Krol, Marc Nolte, Alberto Diaspro, Davide Mazza, Raffaella Magrassi, Alessandra Gliozzi and Andreas Fery,
"Encapsulated Living Cells on Microstructured Surfaces",
American Chemical Society.
Paola Ramoino, Silvia Scaglione, Alberto Diaspro, Francesco Beltrame, Marco Fato, Cesare Usai,
"GABAA receptor subunits identified in Paramecium by immunofluorescence confocal microscoy",
FEMS Microbiology Letters 238 (2004) 449–453.
Chirico G., Cannone F., Baldini G., and Diaspro A.
"Two-photon thermal bleaching of Single Fluorescent Molecules. Biophysical J.", 84 (1) (2003).
A.Diaspro, D.Silvano, S.Krol, O.Cavalleri, A.Gliozzi
"Single living cell encapsulation in nano-organized polyelectrolyte shells", Langmuir, 18, 5047-5050, (2002).
Barboro P., D’Arrigo C., Diaspro A., Mormino M., Alberti I., Parodi S., Patrone E., Balbi C.
"Unravealing the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMa to a Lamin Scaffold. Exp. Cell. Res. 279", 202-218, (2002).
A.Diaspro (ed.)
“Confocal and Two-photon Microscopy: Foundations, Applications and Advances”, Wiley-Liss, Inc, 1-567 (2002).
soon avaible
Diaspro, A; Federici, F
"Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy, Applied Optics", Volume 41, Issue 4, February 1, 685-690 (2002).
A.Diaspro
"Building a two-photon microscope using a confocal laser scanning architecture. "Methods in Cellular Imaging" (A.Periasamy, ed.), Oxford University Press, New York, 162-179, 2001.
soon avaible
T.Pecere, M.V.Gazzola, C.Mucignat, C.Parolin, F.Dalla Vecchia, A.Cavaggioni, G.Basso, A.Diaspro, B.Salvato, M.Carli, G.Palù
Aloe-emodin is a new type of anticancer agent with selective activity against neuroectodermal tumors. Cancer Research, 60 (11), 2800-2804, 2000.
A.Diaspro, Two-photon excitation. A new potential perspective in flow cytometry. , invited review, Minerva Biotecnologica, 11(2), 87-92, 1999. soon avaible
A.Diaspro, M.Aguilar, B.Bianco,
"Detection of Displacements in the Nanometer Scale by Optical Tunneling" , Sensors and Actuators, A ,37/38, pp.557-581, 1993
soon avaible
Diaspro A., M.Bertolotto, L.Vergani, C.Nicolini,
"Polarized Light Scattering of Nucleosomes and Polynucleosomes. In situ and in vitro studies", IEEE Transactions on Biomedical Engineering, BME-38 (7), p.670-678, 1991.
soon avaible